Brand strategy for growth Assignment Example | Topics and Well Written Essays - 1000 words

Brand strategy for growth - Assignment Example Moreover, setting up targets in an organization practically fixes the quantitative target values and aiming in context with the divisional plans identifying the contributions made by each department within the organization. Carrying out performance analysis which includes analyzing and discovering the gap between the planned desired performances enhances performance. The actual reality and final choice of strategy, which is the ultimate step in the formulation, become imperative. Consider organizational goals, organizational strengths, potentials, limitations, as well as the external opportunities (Banerjee, 2007). The brand manager should seek to understand the market by defining it category. An incoming brand manager should have in order to carry out strategy formulation is a better understanding of his/her organization. This is done by carrying out both internal and external analysis of the organization. It helps them get a better glimpse of the organizations strengths, weaknesses and opportunities as well as a better understanding of its competitors. Carry out a SWOT analysis. The endeavor helps the manager be in and advantage position since they are able to know the competitor approaches pricing and models of communication. In addition, the evaluation will unearth the consumer patterns; make a consideration of brand size metrics and loyalty measures (van Gelder, 2004). The management has to check the brand availability with regard to the mantel and physical disposition. The next important aspect an incoming manager needs to be aware of is the status of the brand in question, what is the mental availability of the brand? (Are the customers aware of the brand in question), what is its general buying patterns? (When is its buying seasons? who are the buyers? And the status of its monopoly effect in the market). All these will enable the brand manager to be in a position to better understand his or her brand better and hence help them in coming up

Business ethics Essay Example | Topics and Well Written Essays - 750 words - 2

Business ethics - Essay Example Most of the organizations have defined formal corporate ethics because most of the decisions of organizations are influenced by ethical issues. The employees feel very comfortable when a predefined corporate ethical is present in the organization because they get a clear understanding of what is right and what is wrong. The compliance of employees to corporate ethics is usually ensured by organizations by setting a formal corporate ethics program. Organizations have developed different programs and procedures to enhance the compliance to corporate ethics. For example, at TEPCO, there is a Corporate Ethics Committee consisting of top management, which work closely with ethical lines and establish corporate ethics carefully.1 The diagram (Appendix I) shows that once the corporate ethical conduct is formulated at top management level of TEPCO, it is promoted through incorporating the norms into culture and by revising the regulations and manuals. Moreover, through formal training programs and continuous monitoring, the compliance to corporate ethics is ensured. In this way, formal regulations and cultural norms strengthen the values, thereby, ensuring corporate ethical norms at TEPCO. There are some ethical guidelines, which are defined to develop corporate ethics programs. The ethical guidelines are the guidelines that are established to oversee what is suitable within the quest of specific objectives such as guidelines published by BPS (ITS, 2009). The ethical guidelines of companies usually differ and tend to be more specific. For example, American Mathematical Society has defined few ethical guidelines, which can be categorized into four sections including ethical guidelines related to mathematical research and its presentation, social responsibility of mathematicians, education and granting of degrees and ethical

Streptozotocin (STZ) Induced Diabetes Experiment

Streptozotocin (STZ) Induced Diabetes Experiment MATERIALS AND METHODS 3. MATERIALS AND METHODS 3.1 Animal Selection and Care STZ induced diabetes are commonly performed in Male Wistar Kyoto rats. In these models, male Wistar rats at 8 weeks of age (200–300 g) are selected from animal supply facility of SGPGIMS Lucknow, and house in a 12-h light, 12-h dark cycle environment, temperature 25 °C, with standard rat diet and water in metabolic cages for one week prior to STZ treatments. The study had clearance from Institute animal ethics committee. 3.2 Study Design Wistar Rat (Preparation and Validation of animal model of DN) 50 mg/kg STZ induced Diabetic Rat (n=3) Normal Rat (n=3) After 8th week rat are sacrificed for validation of Diabetic nephropathy 3.3 Induction and Validation of Diabetes by Streptozotocin There is a lack of appropriate animal model that could spontaneously develop DN. It has been a significant limitation to find out specific factors that is underlying this disease and also the development of new therapeutic methods or strategies to prevent progressive renal disease in diabetes (Morcelo A. Nobrega et. al. 2004). Most Sprague-Dawley (SD), Wistar-Kyoto (WKY) rats are used as model for performing STZ-induced diabetic nephropathy. Here, in these models, 8 weeks old male rats (200-250gm) are starved or kept on fasting for 18hrs and after that STZ was injected intraperitoneal (WKY-50mg/kg, SD-55mg/kg and SHR-45mg/kg) with sodium citrate buffer (1ml/kg) (Ma G et. al. 2004 and Cooper ME et. al. 1988). STZ given here intraperitoneally to the rats, however, it is a very less common procedure as intravenous injections are comparatively easy to perform in rats and give more reliable results. In addition to the STZ dosage required for inducing diabetes via an intraperitoneal route is relatively higher as compared to other route. After giving STZ, the rats should be given ample amount of drinking water with sucrose for 48 hrs (15g/L), to avoid early mortality of rats as stored insulin is released from damaged pancreatic Islets of Langerhans. After completion of 1 week of STZ induction, the rats must be assessed for hyperglycaemia and also those with fasting blood glucose of over 280 mg/dl (15 mmol/L), which is usually around 90%, and it should be included in the studies of diabetic nephropathy. In order to prevent subsequent development of ketone uria, subsequent injections of long-acting insulin (approx. 2-4 U/rat) should be given daily to maintain the blood glucose levels in a desirable range(300-600 mg/dl, 16-33 mmol/l) (Davis BJ et. al. 2003). Studies exploring the effects of treatment on the development of DN should not be underway until at least 3 weeks after STZ when the kidneys have improved from the acute mild nephrotoxic effects of STZ (Kraynak AR et . al. 1995) This drug i.e., STZ has been shown to induce a diabetic state in 72 hrs (3 days) as documented by examining tail blood samples using a Glucometer (Optimum Exceed) Diabetic animals and non-diabetic control group were kept in metabolic cages individually and separately and under feeding and metabolism control. Glucose in the blood of diabetic rats exceeded that of the non-diabetic control ones. Food consumption was measured in terms of (gm), water consumption was measured in terms of (ml) and urine volume was measured in terms of (ml) on a daily basis and glucose in blood serum were also measured, so that chemical diabetes was verified in rats injected with Streptozotocin. 3.4 Estimation of Creatinine Modified Jaffe’s method was used for colorimetric estimation of creatinine concentration in urine samples. Principle: Creatinine + picric acid Creatinine picrate (Yellow) (Orange) The resultant orange color is measured colorimetrically. However, the intensity of the resultant orange color is directly proportional to the concentration of creatinine in the sample. Method: Draw the Blood from a vein and then transferred into the vial. Centrifuge the blood for 10 minutes and Serum is obtained. Separate out the serum in different eppendrofs. The concentration of creatinine is calculated in the serum sample using the Jaffe’s method, as follows: In a clean dry test tube add 0.5 ml distilled water (blank) or serum (test), add 0.5 ml NaOH and then 0.5 ml picric acid. Mix all the contents of each tube. Left to stand for 15 minutes. The absorbance is measured at ÃŽ »max 500 nm. If a standard creatinine solution (0.55 mg/dl) has an absorbance value of 0.30, then the concentration of creatinine in the provided serum sample is calculated by using the following equation: Ctest = Cstd x 3.5 Estimation of Urinary Albumin Enzyme linked immunosorbent assay (ELISA) for detection of rat albumin in serum, plasma or urine. Other biological fluids that contain Rat Albumin, such as faeces or saliva, may be suitable samples. 3.5.1 Principle: The antigen present in urine sample are allowed to stick to a poly vinyl and then plate is washed to separate antigen and antibodies from remaining sample components. To this plate a corresponding second antibody is added to get fixed to the already adhered first antigen in the plate. To this added second antibody, an enzyme is also tagged is that, when a suitable substrate is added, the enzyme reacts with it to produce a colour. This colour produced is measurable as a function or quantity of antigen present in the urine sample and there by identified. 3.5.2 Chemical and material required: 96-well plate ELISA Coating Buffer ELISA Wash Solution ELISA Blocking Buffer Sample/Conjugate Diluent (ELISA Blocking Buffer + Tween 20) 10% Tween 20 Enzyme Substrate, TMB ELISA Stop Solution Additional Materials Required: Ultrapure water Precision pipettors, with disposable plastic tips Polypropylene, polyethylene or glass tubes to prepare standard and samples Containers to prepare buffers An aspiration device or an automated 96-well plate washer Disposable reagent reservoirs A standard microtiter plate reader for measuring absorbance at 450 nm 3.5.3 Procedure: Added 100  µl of diluted coating antibody to each well. Samples were run in duplicate. Incubated at room temperature (20-25 C) for 1 hour. Washed plate FIVE times. Added 200  µl of Blocking Solution to each well. Incubated at room temperature for 30 minutes. Washed plate FIVE times. Added 100  µl of standard or sample to well. Incubated at room temperature for 1 hour. Washed plate FIVE times. Added 100  µl of diluted HRP detection antibody to each well. Incubated at room temperature for 1 hour. Washed plate FIVE times. Added 100  µl of TMB Substrate Solution to each well. Developed the plate in the dark at room temperature for 15 minutes. Reaction was stopped by adding 100  µl of Stop Solution to each well. Absorbance was measured on a plate reader at 450 nm. 3.5.4 Precautions: Store all reagents at 2-8 ºC. Do not freeze reagents. All reagents must be at room temperature (20-25  ºC) before use. Vigorous plate washing is essential. Use new disposable pipette tips for each transfer to avoid cross-contamination. Minimize lag time between wash steps to ensure the plate does not become Completely dry during the assay. Avoid microbial contamination of reagents and equipment. Automated plate Washers can easily become contaminated thereby causing assay variability. Take care not to contaminate the TMB Solution. Do not expose TMB Substrate solution to glass, foils, or metal. If the solution is blue before use, do not use it 3.6 Oral Glucose Tolerance Test Rats are fasted overnight (12-16 hours) before the test, sedated rats, will be given 50 % dextrose (3ml/kg body weight) intraperitoneally. Whole blood will be collected from the tail vein at 0, 15, 30, 60, 120, and 180 min after the administered of glucose for the measurement of glucose with a glucometer. 3.6.1 Material Required: Glucometer and glucose strips Dextrose Gauge needles Timer and Pen Table of mice for record keeping of glucose values 3.6.2 Preparations before the test: Weigh the animals before the test. The animals are fasted overnight (approximately 16 hours: 5 pm to 9 am). Place each of the animals in a separate fresh cage with no food, but make sure they have water bottles. Make sure that there is no activity in the animal room before and while you are performing the test. Prepare the glucose solution the night before the test. 3.6.3 Procedure: After the overnight fast, blood glucose was determined (time 0) in a drop of blood as following: Rats were placed on top of the cage (let it hold onto the grid). The tail tip was pricked with a needle, wiped off the tail tip with gauze, and the next drop of blood was used for the determination of glucose with a glucometer. Glucose solution was given orally. Blood glucose was determined at 10, 20, 30, 60, 90 and 120 min after the administration of glucose. For the determination of glucose at each of these time points collected a drop of blood as following: wiped the cut end of the tail to break any blood clot that had formed and collected the next drop of blood. If the blood does not appear spontaneously â€Å"milk† the tail by holding the tail between your thumb and index finger and move along the tail from the base of the tail to the tip of the tail while applying gentle pressure. 3.7 Assessment of Renal Histopathological Injury 3.7.1 Tissue preparation for histology: After 8 weeks the rats were weighed, sacrificed out in accordance to the Institutional animal ethics committee by using suitable anaesthetic agent (Ketamine) and their kidneys were taken out. Left kidneys were perfusion fixed for Histopathological and IHC studies and right kidneys were freezed immediately for western blotting and RT-PCR. Preparation of paraffin blocks of kidney: Kidneys were preserved in Para- formaldehyde is taken out in a glass slab for sectioning. A two cross section of upper half of kidney was done with sterile blade for paraffin embedding. The whole process for preparation of paraffin blocks took two days. Day first: The Formalin fixed kidneys were picked up and place in plastic cassettes was sequentially dipped in alcohol for dehydration. The schedule is as followed: 50% Alcohol →1.5hrs 70%Alcohol →1 hrs. 80% Alcohol →1 hrs. 90% Alcohol →1hrs 100% Alcohol →1hrs (twice) The cassette containing the tissue was left overnight in 100% absolute alcohol. Day Second: On 2nd day we perform the following treatment to tissue containing cassette Cassette was removed from 100% alcohol and dipped for CHCl3 treatments Chloroform (A) 1.5 hours. Chloroform (B) 1.5 hours. Chloroform (C) 1.5 hours. The cassette was then kept in melted paraffin wax (at 58 °C 65 °C in water bath) following two changes of paraffin wax for proper blocking. The steel chocks are kept at the 65 °C electronic heater and the paraffin treated kidney in plastic cassettes are opened and place in pre-heated steel chocks together with melted paraffin wax and closed with cassette. Block was kept at room temperature to solidify the melted wax. The paraffin block containing tissue was sectioned with microtome. The block was fit properly in the Microtome machine 5 µ sections were cut. 3.8 Periodic Schiff’s Acid (PAS) Staining 3.8.1 Principle: PAS (Periodic Acid Schiff) is a method of staining used for the detection of polysaccharides i.e., glycogen and mucosubstances that is glycoprotein’s and glycolipids. PAS stain is a histochemical reaction. In the reaction, periodic acid oxidises vicinal diols in these sugar. It oxidized the aldehyde formed by carbon-carbon bonding that react with fuchsin-sulphurous acid and forms the magenta colour. This periodic acid exposes the glycogen to give a colouring product. The Schiff’s reagent is a very specific agent that only reacts with the carboxylic group compounds. Material required: Glass slides and cover slips Poly Lysine (Sigma Aldrich, USA) Xylene Graduated Alcohol (30%, 50%, 70%, 90% and 100%) Periodic acid Schiff Reagent Haematoxylin Acid alcohol (1% HCl in 70% alcohol) DPX mountant 3.8.3 Protocol for PAS Staining: Keep the Poly-Lysine coated slides on hot plate for 15-20 min. Dip in warm Xylene for 10 min twice. Pass the slide through graded alcohol 100% alcohol- (10 minutes) twice 90% alcohol (5 minutes) 70% alcohol (5 minutes) 50% alcohol (5 minutes) 30% alcohol (5 minutes) Dip in distilled water for 5 min. Place the slide containing section into 0.5 % Periodic acid for 5 minutes. Rinse with distilled water. Schiff Reagent was added for 10 min until deep magenta colour appear. Wash in running tap water for 5 min. Counter stain in haematoxylin Rinse with distilled water. If high stain come, then wash with acid alcohol (1% HCl+70% alcohol) Wash the slide in tap water. Dehydrate in alcohol, and dry the slides. After drying mount the slides by DPX mountant. Masson’s Trichrome Staining 3.9.1 Principle: MT Staining method is used to demonstrate the increase of collagen in diseases. This method of staining uses three dyes of contrasting colours for the selective staining of basic tissue components i.e., muscle, collagen fibers, fibrin, and erythrocytes. The general phenomenon of trichome staining is that smallest dye molecule colours or stains the less porous tissues. However, the penetration of dye of larger molecular size is depends on the expense of small molecules. Some others suggests that the acid dye firstly stained the tissue then the Biebrich Scarlet will binds with the acidophilic components of the tissue, after which when treated with the phosphor acids, the components that are less permeable will retain the red colour, because of the collagen this red colour is pulled out and at the same point of time causing a link with the collagen to bind with the aniline blue. . At the outset it must be made clear that the methods control how ionised acid dyes react with ionised basic tissues. 3.9.2 Material required: Bouin’s solution Xylene Graduated Alcohol (30%, 50%, 70%, 90% and 100%) Weigert’s iron hematoxylin solution Biebrich scarlet acid fusin phosphomolybedic-phosphotungustic acid solution Aniline blue solution 3.9.3 Protocol for MT Staining: Keep the Poly-Lysine coated slides on hot plate for 15-20 min. Dip in warm Xylene for 10 min twice. Pass the slide through graded alcohol 100% alcohol- (10 minutes) twice 90% alcohol (5 minutes) 70% alcohol (5 minutes) 50% alcohol (5 minutes) 30% alcohol (5 minutes) Dip in distilled water for 5 min. For formalin fixed tissue, re-fix in Bouin’s Solution for 30 minutes to 1 hr. at 56 ºC to improve the staining quality although this step is not necessary. Rinse in running tap water for 5-10 minutes to remove the yellow color. Stain in Weigert’s iron hematoxylin working solution for 10 minutes. Rinse in running warm tap water for 10 minutes. Wash in D/W Stain with Biebrich Scarlet Acid Fusin for 5 minutes. Differentiate in phosphophosphomolybedic-phosphotungustic acid solution for 10-15 minutes. Transfer the section directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse directly in D/W and differentiate in 1% Acetic acid solution for 2 minutes. Rinse slides, dehydrate through Alcohol Clean slides in xylene Mount with DPX mountant. NoteNuclei-Black, Muscle Fibre-Red, Collagen-Blue, Cytoplasm-Red Modified Immunohistochemistry 3.10.1 Principle: Immunohistochemistry (IHC) is the process whereby antibodies are used to detect proteins (antigens) in cells within a tissue section (for instance liver, pancreas or the heart). Immunohistochemistry exploits the principle that in biological tissues antibody binds to the specific antigens. This tool is used to localize specific antigens in tissue sections with labelled antibodies based on antigen-antibody interactions. This antigen-antibody interaction can be visualized in number of ways i.e., the immune reactive products can be visualized by a marker including fluorescent dyes, enzymes in general; radioactive elements or colloidal gold. This IHC technique is widely used by the researcher in basic research for understanding the differentially expressed proteins and for the localization and distribution of biomarkers in different- different parts of biological tissues. 3.10.2 Material required: Poly-Lysine coated slide. Xylene 1X- PBS buffer.(Sigma Aldrich Inc., USA) Graduated Alcohol (30%, 50%, 70%, 90% and 100%) DPX mountant for microscopy Acid alcohol (1% HCl in 70% alcohol) citrate buffer Sodium Borohydrate Hydrogen Peroxide Blocking solution Primary antibody Secondary antibody Streptovidin HRP 3.10.3 Protocol for Modified Immunohistochemistry: Cut the section of 3 to 5  µm. Warm the slide on hot plate (55 °c) for 30’. Dip the slide in warm Xylene twice for 10 minutes. Wash the slides three times in TBS or PBS for 5 minutes each. Dehydration using 100% Alcohol twice for 10 minutes each and 90, 80, 40, 50 and 30 percent for 5’ each in coupling jar. Wash thrice in TBS or PBS for 5 minutes each. Antigen retrieval -10 minutes for 98 °c in citrate buffer pH-6 (Note: 0.294 gm in 100 ml MQ with pH=6, 1 10mm citrate buffer) Here we are using pressure cooker for Antigen retrieval Cool the citrate Buffer slides. Give the Sodium Borohydrate treatment in coupling jar (To remove Background). (Dark Condition) (Note:- 1% NaBH4) Wash slides thrice in TBS or PBS for 5 minute each. Hydrogen Peroxide Blocking (3% H2O2 in Methanol or water –Dip the slides in it and keep it for 30’). Wash thrice in TBS or PBS for 5 minutes each. Blocking solution[0.3% Triton X and 5% sheep serum in 1xPBS or 1xTBS] For 2ml (6 µl Triton, 100  µl sheep serum, 19 of 94  µl (1xPBS or 1xTBS)) 14. Blocking for 30minutes at 37 °c in lab (some time 1hour also) Wash the slides thrice in TBS or PBS for 5’ each. Primary antibody in TBS or PBS solution, left overnight at 4 °c (Note: 1:200 dilution for fibronectin 1:500 dilution of Collagen IV). Wash the slides thrice in 1xTBS or 1xPBS Secondary antibody added on slide and keep it for 30’ to 1hour. But parafilm on it. (Note: 1:200 dilution) Wash the slide thrice in 1xTBSor 1XPBS for 5 minutes. Streptovidin HRP (1:200) in 1xTBS or 1xPBS for 20 minutes at Room temperature. Three wash in 1xTBS 5 minutes each. DAB (Till color in Brown) (15 minutes) 23.1xPBS (5minutes) wash 24. Counterstain for 30 seconds with Hematoxylene (Directly put on slide) 25. 1xTBS or PBS immediate wash 26. Dehydrate and dry and mount

Inertia :: essays research papers

Centuries of thought and experimentation by several of the greatest minds to ever walk the earth have contributed to the awareness of this property of matter known as Inertia. It all began with the investigation of an ancient Greek scientist and philosopher by the name of Aristotle. Through countless observations and hypotheses, Aristotle grew almost certain of a few things involving motion. Aristotle’s findings suggested mainly that Earthly objects seek their natural place at rest. This natural place related to earth, water, air and fire respectably. He also believed that motion must be subjected to unnatural force, meaning motion could not come about without it. In order to maintain this motion, Aristotle stated that force must be applied to the object constantly. These four statements were considered concrete in the world of physics for nearly two thousand years after Aristotle’s passing. It would take an Italian physicist and astronomer named Galileo to eventually disprove his thoughts on motion. Galileo proposed his formulation of the concept of inertia, which is the property of matter that causes it to resist any change of its motion in either direction or speed. In other words, if an object in a state of motion possesses an â€Å"inertia†, it causes the object to remain in that state of motion unless an external force acts on it. Galileo figured this out by first meticulously observing Aristotle’s concept of motion. By virtue of a series of experiments, Galileo realized that the analysis of Aristotle was incorrect because it failed to account properly for a hidden force, the frictional force between the surface and the object. For example if one were to push a block of wood across a table, there would be two opposing forces that act, one of them being the force associated with the push and the other, the force that is associated with the friction, which acts in the opposite direction. Galileo realized as the frictional forces were decreased the object wo uld move further and further before stopping. An English mathematician and physicist by the name of Sir Issac Newton would later develop Galilieo’s idea of inertia into one of his three scientific laws. Aware of other factors that played a roll in the theory of inertia, Newton proposed two other laws that supported it. They are included in his famous three laws of motion. The first law of motion states "a body in motion shall remain in motion moving with a constant speed and direction, unless acted on by an outside force".

Individual vs. the Society in “The Scarlet Letter” Essay

In The Scarlet Letter by Nathaniel Hawthorne, the theme of the individual versus society is prevalent. One of the most intriguing characters in the novel is Hester Prynne, who is ostracized by the society around her. Hawthorne uses symbols to accentuate how Hester chooses to accept her branded punishment as a moral obligation rather than a mark of shame. Her individualism is achieved through a clear conscience and accepting the fact that she is unique, distanced from the Puritans surrounding her. Immediately, The Scarlet Letter sets up a clear contrast between Hester and the other ladies in Boston, Massachusetts. Hester emerges from the prison as a gorgeous woman who was, â€Å"tall, with a figure of perfect elegance on a large scale. She had dark and abundant hair, so glossy that it threw off the susnhine with a gleam, and a face which ,beside being beautiful from regularity of feature and richness of complexion†¦ was ladylike, too, after the manner of the feminine gentility of those days; characterized by a certain state and dignity.† (Narrator, p. 46-47) Meanwhile, the other women in Boston are portrayed as gossiping quacks who ridicule Hester out of jealousy and spite. Hester is further distanced from the mob throng through her elegant garb and her skill of needle-work. While on the scaffold, and later when she moves into a cottage distanced from everyone else, Hester remains defiant. She asserts her quasi rebellious personality by not wavering amid the penetrating stares of the townspeople. In addition, she chooses to stay in Boston rather than take the opportunity to escape and start a new life. By running away, Hester would be acknowledging society’s power of her. Instead, she desires to establish her own identity and not have society determine it for her. If Hester were to succumb to outside pressure, it would further undermine what little integrity the townspeople see in her. However, the cruel taunts are meaningless to her, because Hester is adamant in her convictions; she has a genuine purpose in life. Her daughter Pearl is an important part of Hester’s life. Much more than a living embodiment of the sin that Hester is reminded of through the scarlet letter, Pearl allows Hester to have a reason to live and a reason to stay. Pearl acts as a reality check, for she daily reminds her mother of her sin. Yet, Pearl is a miracle child, by proving that beauty can spring from sin. She offers Hester a mirror of herself, and an enduring obligation for which to uphold. In addition, Hester feels an obligation to stay in New England because she feels morally bound to stay with Pearl’s father. The scarlet letter in a sense defines who Hester is. Lavishly decorated â€Å"in fine red cloth surrounded with an elaborate embroidery and fantastic flourishes of gold thread,† (Narrator, p. 46) the letter â€Å"A† binds Hester to serve her due atonement for the wrongs she has committed. However, her brilliant skills at needle-work and uniqueness of individuality are frowned upon by the society. Out of malice and jealousy, they verbally abuse both her and Pearl, degrading them to worthless figures while they themselves are hypocrites in their own right. Ironic takes its finest form when the skillful works of Hester are adorned by those highest officials that are punishing her. Despite the ridicule, Hester stands tall. She knows the expectations that are placed on her, and that the shame incurred by her will extend for possibly generations to come. Hester is not willing to mold herself to the eager hands of others so that they may satiate their cruel desire to see her torture in her mental prison. She strives for compassion and well-being amid all the harsh words. This is evidenced in her treatment of Pearl, who is exalted far beyond the other children. She is dressed in the most extravagant attire, while being taught heavenly virtues. Motherly love defies all pressures, and Hester’s undeniable love for Pearl is the quintessence of this. In the strict morals of Puritan society that are delineated in The Scarlet Letter, Hester Prynne undergoes many tribulations. Her wrongdoing helps provoke the angry sentiments of the townspeople, who are determined to see her suffer to the fullest extent. However, Hester’s attitude is one of moral obligation and compassionate defiance. She retorts with nothing but love, as beautifully demonstrated in her treatment of Pearl. Hester is unique, and so  is her unwavering attitude regardless of what the rest of society thinks of her. She answers to a higher order and never relents her conscience to the greedy hands of others.

Harlem Renaissance Poem Analysis Paper

Lee-Michael Torcedo Prof. Quiroz English 1302 October 22, 2012 Dreams After the civil war, African Americans obtained their freedom. Still despised by many white Americans, African Americans continued to fight for justice. Around the early 1900’s the Harlem Renaissance began across the nation they fought for their culture and expressed it through art, music, dance, and literature. One of the biggest names in the Harlem Renaissance is Langston Hughes (Harlem). The poems he wrote better expressed the feelings of the many African Americans during this era.Langston Hughes published his poem, â€Å"As I Grew Older,† which explains the difficulty many African Americans had with following their dreams during this time. On February 1, 1902, Carrie M. Langston and James N. Hughes gave birth to, arguably, the most famous Harlem Renaissance literary poet, Langston Hughes. He grew up attending school in Kansas and Illinois, and graduated from High School in Ohio where he began writ ing his poetry. Later, Hughes went on to college, but stopped shortly after.He took many jobs in New York and a job working on a freighter that took him across the Atlantic Ocean to African, Spain, and France. He returned to Washington D. C. to live with his mother with hopes of returning to college. He had trouble making enough money and didn’t go back to college until a year later where he graduated from Lincoln University in 1929. After college, he began publishing his works and started to become famous. On May 22, 1967, Langston Hughes died of cancer in New York (Langston). Langston Hughes’ legend still lives to this day, and his poems are often mentioned in literature.In his poem â€Å"As I Grew Older,† published in 1925, he begins by saying â€Å"It was a long time ago/ I have almost forgotten my dream/ But it was there then/ In front of me/ Bright like a sun—/ My dream† (1-4). Whether or not Langston Hughes wrote this about himself or not, i t can go either way on who could be mentioning this and who is reflecting on the past that was a â€Å"long time ago†. Hughes gave the perspective that the main persona is talking about his past and about how even though his dream was right in front of him, he could not grasp it.In the early ages of Langston Hughes life, many obstacles barricaded him from reaching his dreams and goals, losing sights of what he really wanted. He talks about a wall in the poem that rises slowly between him and this dream. It â€Å"rose until it touched the sky† (11). The wall symbolizes his obstacles or what blocked him from his dreams. The wall rising until it touched the sky showed the degree and emphasis of the difficulty for Hughes to see his dreams. On line thirteen he mentions the shadow that the wall covered him in. There was no more light to be seen.All hope seemed to be lost until he â€Å"breaks† down the wall, â€Å"shatters† the darkness, and â€Å"smashes† the night. At the other side of this wall, is light, the sun, and his dream. Langston Hughes must have at one point in following his dream felt so overwhelmed with everything in his way and just â€Å"lie down in the shadow† (15). Just like in his poem, he takes down that wall and does not let it get in his way. He is successful in reaching his dream of becoming a well-known poet even through the worst of times. His poem reflects the situation many African Americans faced during this time.Freedom is still new to most and following dreams is difficult because of prejudism. Many African Americans ran into this â€Å"wall† and many did not break through as Langston Hughes did. Although some did, they went through the same difficulties as Hughes poetically explained in his poem. The beauty of this poem is the universality of it. It may have been written to explain the difficulties African Americans had, but it reflects the difficulty for anyone following their dreams sh ould not be forgotten. They are not always easy to achieve, but that does not mean one should give up.The poem is powerful in meaning and motivational as it speaks to anyone and illustrates that you should never surrender. Langston Hughes’ dream as a young boy was to be known as a poet. Much like the wall, he had to overcome many obstacles to achieve his dreams. He would vision his dream so clearly in front of him like the sun but as he got older and taller, so did this wall that blocked his way. Even though, Hughes smashed down that wall and become one of the most well-known founders of the Harlem Renaissance. Works Cited â€Å"Harlem Renaissance – Biography. com – Biography. com. †Ã‚  Famous Biographies & TV Shows – Biography. om. N. p. , n. d. Web. 22  Oct. 2012. . Hughes, Langston. â€Å"As I Grew Older by Langston Hughes. †Ã‚  PoemHunter. Com – Thousands of poems and poets. Poetry Search Engine. N. p. , 3  Jan. 2003. Web. 22à ‚  Oct. 2012. . â€Å"Langston Hughes Biography – life, children, parents, name, story, history, school, mother, book, information, born, college. †Ã‚  Encyclopedia of World Biography. N. p. , n. d. Web. 22  Oct. 2012. .